中国组织工程研究 ›› 2014, Vol. 18 ›› Issue (3): 395-400.doi: 10.3969/j.issn.2095-4344.2014.03.011

• 药物控释材料 drug delivery materials • 上一篇    下一篇

骨形态发生蛋白2/聚乳酸缓释微球的制备及表征

马立坤,叶  鹏,黄文良,田仁元,邓  江   

  1. 遵义医学院第三附属医院骨一科,贵州省遵义市  563000
  • 出版日期:2014-01-15 发布日期:2014-01-15
  • 通讯作者: 邓江,主任医师,研究生导师,遵义医学院第三附属医院骨科,贵州省遵义市 563000
  • 作者简介:马立坤,男,1984年生,湖南省武冈市人,汉族,遵义医学院在读硕士,医师,主要从事骨与关节创伤方面的研究。
  • 基金资助:

    贵州省省长基金项目(2011(25)),项目名称:rhBMP-2缓释系统/自体松质骨复合生物支架修复骨缺损的研究;贵州省科学技术基金项目(黔科合SY[2010]3101),项目名称:SF-CS-nHA支架与基因修饰后BMSCs构建人工骨种植体的成骨作用研究;遵义市科技局、遵义医院联合科研项目(遵义市科合社字[2010]015号),项目名称:RGD表面修饰SF/CS/n-HA材料复合携带目的基因的BMSCs成骨性能研究

Preparation and characterization of recombinant human bone morphogenetic protein-2/ poly lactic acid sustained release microspheres

Ma Li-kun, Ye Peng, Huang Wen-liang, Tian Ren-yuan, Deng Jiang   

  1. First Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • Online:2014-01-15 Published:2014-01-15
  • Contact: Deng Jiang, Chief physician, Master’s supervisor, First Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • About author:Ma Li-kun, Studying for master’s degree, Physician, First Department of Orthopedics, Third Affiliated Hospital of Zunyi Medical University, Zunyi 563000, Guizhou Province, China
  • Supported by:

    the Governor Fund of Guizhou Province, No. 2011(25); the Scientific Foundation of Guizhou Province, No. SY[2010]3101; the Joint Scientific Research Program of Guizhou Science and Technology Bureau and Zunyi Hospital, No. [2010]015

摘要:

背景:聚乳酸具有良好的生物相容性,是优良的药物缓释载体。
目的:制备重组人骨形态发生蛋白2/聚乳酸缓释微球,考察其理化特性。
方法:采用复乳溶剂挥发法制备重组人骨形态发生蛋白2/聚乳酸缓释微球,进行扫描电镜、激光粒度、Zeta电位、溶胀性能检测及采用ELISA试剂盒检测包封率、载药率及体外释药率。
结果与结论:扫描电镜见重组人骨形态发生蛋白2/聚乳酸缓释微球微球近似圆形,形态较规则,分散性较好,表面光滑。激光粒度分析重组人骨形态发生蛋白2/聚乳酸缓释微球微平均粒径839.6 nm,Zeta电位(-32.93±3.74)mV,微球溶胀系数1.157±0.059,包封率及载药率分别为(88.943±2.878)%,(0.026±0.001)%;微球在第1天释药约10.199%,随后释药较恒定,至第19天累计释药率为54.643%。说明制备出的重组人骨形态发生蛋白2/聚乳酸缓释微球的粒径达到中华人民共和国药典第10版二部关于亚微球的定义标准及包封率不低于80%的要求,并且在体外具有很好的缓释功能。


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

关键词: 生物材料, 缓释材料, 重组人骨形态发生蛋白2, 聚乳酸, 缓释微球, 包封率, 载药率

Abstract:

BACKGROUND: Poly lactic acid as an excellent delivery has good biocompatibility.
OBJECTIVE: To prepare recombinant human bone morphogenetic protein-2 (rhBMP-2)/poly lactic acid (PLA) sustained release microspheres, and to study its physical and chemical properties.
METHODS: The rhBMP-2/PLA sustained release microspheres were prepared using w/o/w solvent evaporation method. Scanning electron microscopy, laser particle size, zeta potential, and swelling properties were detected. ELISA kit was utilized for measurement of encapsulation efficiency, drug-loading rate and in vitro drug release rate.
RESULTS AND CONCLUSION: Under the scanning electron microscope, rhBMP-2/PLA sustained release microspheres were approximately circle with excellent dispersion. The uniform spheres were visible with a mean particle size of 839.6 nm. The zeta potential were (-32.93±3.74) mV. The swelling coefficient was 1.157±0.059. The drug-loading rate and encapsulation efficiency of rhBMP-2/PLA sustained release microspheres were (88.943±2.878)% and (0.026±0.001)% respectively. The drug release rate at 1 day was about 10.199%, then the drug release was relatively constant, and till 19 days, the cumulative drug release rate was 54.643%. These findings indicate that the constructed rhBMP-2/PLA sustained release microspheres meet the requirement of the Chinese Pharmacopoeia (10th edition) that the encapsulation efficiency is not less than 80% and the microspheres have a good slow-release function in vitro.


中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程


全文链接:

Key words: biocompatible materials, bone morphogenetic proteins, lactic acid, microspheres, delayed-action preparations

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